Protease Inhibitor Cocktail (200X) from Cell Signaling Technology

Product Specs
ItemProtease Inhibitor Cocktail (200X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7012L
ApplicationsChIP, C&R
Quantity750 µl
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Central nervous system sulfatide deficiency as a causal factor for bladder disorder in Alzheimer's diseaseClinical and Translational MedicineJuly 21, 2023Sijia He, Shulan Qiu, Meixia Pan, Juan P. Palavicini, Hu Wang, Xin Li, Anindita Bhattacharjee, Savannah Barannikov, Kevin F. Bieniek, Jeffrey L. Dupree, et al.light/dark cycles with free access to food and water. Mice were fed Teklad laboratory diet (ENVIGO, Cat. #7012). CST fl/fl mouse was generated as described in our previous study 18 by flanking exon 3 and 4 of the

(2) 1α, 25-Dihydroxyvitamin D3 protects gastric mucosa epithelial cells against Helicobacter pylori-infected apoptosis through a vitamin D receptor-dependent c-Raf/MEK/ERK pathwayPharmaceutical BiologyDecember 31, 2022Shuai Zhao, Daihong Wan, Yaoyao Zhong, Xiwei Xufrom Thermo Fisher Scientific (Waltham, MA, USA). RIPA buffer (#9806), protease inhibitor cocktail (#7012) and phosphatase inhibitor cocktail (#5870) were purchased from Cell Signalling Technology. Cell and

(3) RAD18 opposes transcription-associated genome instability through FANCD2 recruitmentPLoS geneticsDecember 1, 2022Wells JP, Chang EY, Dinatto L, White J, Ryall S, Stirling PC.cells down into 1 ml ice-cold 1X Buffer A (in 500 μM DTT and 1 x protease inhibitor cocktail (PIC) (CST#7012) per IP prep in sonication tubes. Nuclei were pelleted and resuspended in 300 ul of 1 x ChIP buffer

(4) Integrative analysis and prediction of human R-loop binding proteinsG3 Genes|Genomes|GeneticsJuly 29, 2022Arun Kumar, Louis-Alexandre Fournier, Peter C Stirlingscraped into 0.5 ml ice-cold 1× Buffer A [with 500 µM DTT and 1× protease inhibitor cocktail (PIC) (CST#7012) per IP prep in sonication tubes]. Nuclei were pelleted and resuspended in 150 µl of 1× ChIP buffer

(5) Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitinationInternational Journal of Biological SciencesMay 16, 2022Xiaofeng Dai et al.Biotechnology, Catalog# P0013B) supplemented with Protease Inhibitor Cocktail (200×, Cell Signaling Technology, #7012) and Phosphatase Inhibitor Cocktail (100×, Cell Signaling Technology, #5870) for 1 min on ice followed

(6) A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's diseaseDisease models & mechanismsMarch 1, 2022Li ZF, Cui L, Jin MM, Hu DY, Hou XG, Liu SS, Zhang X, Zhu JH.(TBS; 50 mM Tris, 150 mM NaCl, pH 7.5) supplemented with 1% Triton X-100, protease inhibitor cocktail (7012L, Cell Signaling Technology) and 1 mM phenylmethane sulfonyl fluoride (PMSF; ST506-2, Beyotime). After

(7) SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathwayVirologyMarch 1, 2022Xu H, Akinyemi IA, Chitre SA, Loeb JC, Lednicky JA, McIntosh MT, Bhaduri-McIntosh S.(Thermo Scientific, Cat. 87787) in the presence of 1 × protease inhibitor cocktail (Cell Signaling, #7012) for 15min followed by centrifugation (14,000 rpm) at 4 °C for 5 min. Of pre-cleared cell lysates, 5%

(8) Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by AutophagyAdvanced science (Weinheim, Baden-Wurttemberg, Germany)January 1, 2022Yang D, Wu X, Wang W, Zhou Y, Wang Z.87788) containing a protease and phosphatase inhibitor cocktail (Cell Signaling Technology, Catalog # 7012). The protein lysates were precleared with mouse IgG (Sepharose Bead Conjugate; Cell Signaling Technology;

(9) Genome-Wide Chromatin Analysis of FFPE Tissues Using a Dual-Arm Robot with Clinical PotentialCancers (Basel)April 28, 2021Syuzo Kaneko et al.(PMSF; Sigma-Aldrich, P7626), and 1× Protease Inhibitor Cocktail (1× PIC; Cell Signaling Technology, #7012). Samples were sonicated using Bioruptor II (BM Equipment, BR2006A) for 30 min at high 30-s ON and 30-s

(10) Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancerCell death & diseaseFebruary 19, 2021Wang Y, Sun Y, Shang C, Chen L, Chen H, Wang D, Zeng X.immunofluorescence Cells were lysed in RIPA buffer in the presence of PMSF and protease inhibitor cocktail (#7012; Cell Signaling Technology). Protein concentrations were quantified by using BCA protein assay reagent

(11) BRD4 inhibition by JQ1 prevents high-fat diet-induced diabetic cardiomyopathy by activating PINK1/Parkin-mediated mitophagy in vivoJournal of molecular and cellular cardiologyDecember 1, 2020Mu J, Zhang D, Tian Y, Xie Z, Zou MH.containing mitochondria was washed in isolation buffer supplemented with protease inhibitor cocktail (#7012, CST), and was collected for further use after spinning down at 12,000g. 2.9. Measurement of mitochondrial

(12) Novel replisome-associated proteins at cellular replication forks in EBV-transformed B lymphocytesPLoS pathogensDecember 1, 2019Xu H, Perez RD, Frey TR, Burton EM, Mannemuddhu S, Haley JD, McIntosh MT, Bhaduri-McIntosh S.presence of 0.5 μl 1M DTT (Cell Signaling, #7006) and 1X protease inhibitor cocktail (Cell Signaling, #7012); on ice for 15min. Samples were centrifuged (2000g, 5min) at 4˚C, washed once in 1 ml of buffer B (Cell

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Protease Inhibitor Cocktail (200X) from Cell Signaling Technology

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Protease Inhibitor Cocktail (200X) from Cell Signaling Technology

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